Expression of thioredoxin and glutaredoxin in experimental hepatocellular carcinoma-Relevance for prognostic and diagnostic evaluation.
Identifieur interne : 000267 ( Main/Exploration ); précédent : 000266; suivant : 000268Expression of thioredoxin and glutaredoxin in experimental hepatocellular carcinoma-Relevance for prognostic and diagnostic evaluation.
Auteurs : Nabil M. Abdel-Hamid [Égypte] ; Tarek K. Mahmoud [Égypte] ; Shimaa A. Abass [Égypte] ; Mamdouh M. El-Shishtawy [Égypte]Source :
- Pathophysiology : the official journal of the International Society for Pathophysiology [ 0928-4680 ] ; 2018.
Abstract
Hepatocellular carcinoma (HCC), represents more than 85% of liver cancers. The diagnosis of HCC may be delayed due to the absence of early, sensitive and specific biomarkers. This study was conducted to investigate whether the expression of thioredoxin (Trx) and glutaredoxin (Grx) is helpful for HCC diagnosis in an experimental model. Twenty male albino rats were equally divided into two groups (HCC and control). Hepatocarcinogenesis was performed by single intraperitoneal (i.p) injection of 200 mg/kg of diethylnitrosamine (DENA). Two weeks later, 0.05% of phenobarbital (PB) was supplied in the drinking water for other 14 weeks. HCC was diagnosed by measuring serum alpha-fetoprotein (AFP) level and histopathological examination. Our results found that hepatic indices alanine transaminase (ALT), aspartate transaminase (AST), total bilirubin were elevated but decreased total protein level. Lipid peroxidation was elevated through increasing hepatic content of MDA with decreased antioxidant parameters like hepatic SOD, CAT activities and GSH. The current study also found that Trx and Grx tissue genes were overexpressed in HCC group significantly, compared to control group. This study substantiated that increased expression of these enzymes may be predictive of outcomes in HCC.
DOI: 10.1016/j.pathophys.2018.08.008
PubMed: 30224102
Affiliations:
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Abdel-Hamid</name><affiliation wicri:level="1"><nlm:affiliation>Biochemistry Department, Faculty of Pharmacy, Kafrelsheikh University, Kafrelsheikh, 33516, Egypt. Electronic address: nabilmohie@yahoo.com.</nlm:affiliation><country xml:lang="fr">Égypte</country><wicri:regionArea>Biochemistry Department, Faculty of Pharmacy, Kafrelsheikh University, Kafrelsheikh, 33516</wicri:regionArea><wicri:noRegion>33516</wicri:noRegion></affiliation></author><author><name sortKey="Mahmoud, Tarek K" sort="Mahmoud, Tarek K" uniqKey="Mahmoud T" first="Tarek K" last="Mahmoud">Tarek K. Mahmoud</name><affiliation wicri:level="1"><nlm:affiliation>Biochemistry Department, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, 33516, Egypt.</nlm:affiliation><country xml:lang="fr">Égypte</country><wicri:regionArea>Biochemistry Department, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, 33516</wicri:regionArea><wicri:noRegion>33516</wicri:noRegion></affiliation></author><author><name sortKey="Abass, Shimaa A" sort="Abass, Shimaa A" uniqKey="Abass S" first="Shimaa A" last="Abass">Shimaa A. Abass</name><affiliation wicri:level="1"><nlm:affiliation>Biochemistry Department, Faculty of Pharmacy, Kafrelsheikh University, Kafrelsheikh, 33516, Egypt.</nlm:affiliation><country xml:lang="fr">Égypte</country><wicri:regionArea>Biochemistry Department, Faculty of Pharmacy, Kafrelsheikh University, Kafrelsheikh, 33516</wicri:regionArea><wicri:noRegion>33516</wicri:noRegion></affiliation></author><author><name sortKey="El Shishtawy, Mamdouh M" sort="El Shishtawy, Mamdouh M" uniqKey="El Shishtawy M" first="Mamdouh M" last="El-Shishtawy">Mamdouh M. El-Shishtawy</name><affiliation wicri:level="1"><nlm:affiliation>Biochemistry Department, Faculty of Pharmacy, Mansura University, Mansura, 35516, Egypt.</nlm:affiliation><country xml:lang="fr">Égypte</country><wicri:regionArea>Biochemistry Department, Faculty of Pharmacy, Mansura University, Mansura, 35516</wicri:regionArea><wicri:noRegion>35516</wicri:noRegion></affiliation></author></analytic><series><title level="j">Pathophysiology : the official journal of the International Society for Pathophysiology</title><idno type="ISSN">0928-4680</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Hepatocellular carcinoma (HCC), represents more than 85% of liver cancers. The diagnosis of HCC may be delayed due to the absence of early, sensitive and specific biomarkers. This study was conducted to investigate whether the expression of thioredoxin (Trx) and glutaredoxin (Grx) is helpful for HCC diagnosis in an experimental model. Twenty male albino rats were equally divided into two groups (HCC and control). Hepatocarcinogenesis was performed by single intraperitoneal (i.p) injection of 200 mg/kg of diethylnitrosamine (DENA). Two weeks later, 0.05% of phenobarbital (PB) was supplied in the drinking water for other 14 weeks. HCC was diagnosed by measuring serum alpha-fetoprotein (AFP) level and histopathological examination. Our results found that hepatic indices alanine transaminase (ALT), aspartate transaminase (AST), total bilirubin were elevated but decreased total protein level. Lipid peroxidation was elevated through increasing hepatic content of MDA with decreased antioxidant parameters like hepatic SOD, CAT activities and GSH. The current study also found that Trx and Grx tissue genes were overexpressed in HCC group significantly, compared to control group. This study substantiated that increased expression of these enzymes may be predictive of outcomes in HCC.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">30224102</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0928-4680</ISSN><JournalIssue CitedMedium="Print"><Volume>25</Volume><Issue>4</Issue><PubDate><Year>2018</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Pathophysiology : the official journal of the International Society for Pathophysiology</Title><ISOAbbreviation>Pathophysiology</ISOAbbreviation></Journal><ArticleTitle>Expression of thioredoxin and glutaredoxin in experimental hepatocellular carcinoma-Relevance for prognostic and diagnostic evaluation.</ArticleTitle><Pagination><MedlinePgn>433-438</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0928-4680(18)30101-9</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.pathophys.2018.08.008</ELocationID><Abstract><AbstractText>Hepatocellular carcinoma (HCC), represents more than 85% of liver cancers. The diagnosis of HCC may be delayed due to the absence of early, sensitive and specific biomarkers. This study was conducted to investigate whether the expression of thioredoxin (Trx) and glutaredoxin (Grx) is helpful for HCC diagnosis in an experimental model. Twenty male albino rats were equally divided into two groups (HCC and control). Hepatocarcinogenesis was performed by single intraperitoneal (i.p) injection of 200 mg/kg of diethylnitrosamine (DENA). Two weeks later, 0.05% of phenobarbital (PB) was supplied in the drinking water for other 14 weeks. HCC was diagnosed by measuring serum alpha-fetoprotein (AFP) level and histopathological examination. Our results found that hepatic indices alanine transaminase (ALT), aspartate transaminase (AST), total bilirubin were elevated but decreased total protein level. Lipid peroxidation was elevated through increasing hepatic content of MDA with decreased antioxidant parameters like hepatic SOD, CAT activities and GSH. The current study also found that Trx and Grx tissue genes were overexpressed in HCC group significantly, compared to control group. This study substantiated that increased expression of these enzymes may be predictive of outcomes in HCC.</AbstractText><CopyrightInformation>Copyright © 2018 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Abdel-Hamid</LastName><ForeName>Nabil M</ForeName><Initials>NM</Initials><AffiliationInfo><Affiliation>Biochemistry Department, Faculty of Pharmacy, Kafrelsheikh University, Kafrelsheikh, 33516, Egypt. Electronic address: nabilmohie@yahoo.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mahmoud</LastName><ForeName>Tarek K</ForeName><Initials>TK</Initials><AffiliationInfo><Affiliation>Biochemistry Department, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, 33516, Egypt.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Abass</LastName><ForeName>Shimaa A</ForeName><Initials>SA</Initials><AffiliationInfo><Affiliation>Biochemistry Department, Faculty of Pharmacy, Kafrelsheikh University, Kafrelsheikh, 33516, Egypt.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>El-Shishtawy</LastName><ForeName>Mamdouh M</ForeName><Initials>MM</Initials><AffiliationInfo><Affiliation>Biochemistry Department, Faculty of Pharmacy, Mansura University, Mansura, 35516, Egypt.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>09</Month><Day>12</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Pathophysiology</MedlineTA><NlmUniqueID>9433813</NlmUniqueID><ISSNLinking>0928-4680</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Diagnosis</Keyword><Keyword MajorTopicYN="N">Experimental</Keyword><Keyword MajorTopicYN="N">Glutaredoxin</Keyword><Keyword MajorTopicYN="N">Hepatocellular carcinoma</Keyword><Keyword MajorTopicYN="N">Thioredoxin</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>06</Month><Day>05</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2018</Year><Month>08</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>08</Month><Day>31</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>9</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>9</Month><Day>19</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>9</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30224102</ArticleId><ArticleId IdType="pii">S0928-4680(18)30101-9</ArticleId><ArticleId IdType="doi">10.1016/j.pathophys.2018.08.008</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Égypte</li></country></list><tree><country name="Égypte"><noRegion><name sortKey="Abdel Hamid, Nabil M" sort="Abdel Hamid, Nabil M" uniqKey="Abdel Hamid N" first="Nabil M" last="Abdel-Hamid">Nabil M. Abdel-Hamid</name></noRegion><name sortKey="Abass, Shimaa A" sort="Abass, Shimaa A" uniqKey="Abass S" first="Shimaa A" last="Abass">Shimaa A. Abass</name><name sortKey="El Shishtawy, Mamdouh M" sort="El Shishtawy, Mamdouh M" uniqKey="El Shishtawy M" first="Mamdouh M" last="El-Shishtawy">Mamdouh M. El-Shishtawy</name><name sortKey="Mahmoud, Tarek K" sort="Mahmoud, Tarek K" uniqKey="Mahmoud T" first="Tarek K" last="Mahmoud">Tarek K. Mahmoud</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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